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  New type of POU domain in germ line-specific protein Oct-4.

Schoeler, H., Ruppert, S., Suzuki, N., Chowdhury, K., & Gruss, P. (1990). New type of POU domain in germ line-specific protein Oct-4. Nature, 344(6265), 435-439. doi:10.1038/344435a0.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0E16-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-E4D3-5
Genre: Journal Article

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 Creators:
Schoeler, H.1, Author
Ruppert, S., Author
Suzuki, N.2, Author              
Chowdhury, K.2, Author              
Gruss, P.2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Department of Molecular Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578585              

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 Abstract: Members of a family of murine octamer-binding proteins interact specifically with the octamer motif, a transcription regulatory element found in the promoter and enhancer regions of many genes. Oct-4 is a maternally expressed protein that is also present in the pre-implantation mouse embryo. Although many regulatory proteins are expressed in post-implantation embryos, transcription factors regulating pre-implantation processes have remained elusive. The Oct-4 gene is therefore a prime candidate for an early developmental control gene. Here we report the complementary DNA cloning of the mouse Oct-4 gene, and the characterization of the encoded protein(s) by sequential in vitro transcription, translation, DNA-binding and protease-clipping analysis. Deletion analysis shows that the DNA-binding activity is mediated by a POU domain encoded in an open reading frame corresponding to a 324-amino-acid protein. Sequence comparison with known POU domains reveals that Oct-4 is a novel member of the POU-family.

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Language(s): eng - English
 Dates: 1990-03-29
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/344435a0
ISI: A1990CW47800064
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 344 (6265) Sequence Number: - Start / End Page: 435 - 439 Identifier: ISSN: 0028-0836