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  Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene.

Preuss, K. D., Krauhs, E., & Scheit, K. H. (1990). Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Biological Chemistry Hoppe-Seyler, 371(3), 215-222.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0E2E-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3850-4
Genre: Journal Article

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 Creators:
Preuss, K. D.1, Author              
Krauhs, E.1, Author              
Scheit, K. H.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: A synthetic DNA, carrying the coding sequence for seminalplasmin (SAP), the major basic protein of bull semen, was cloned into the C-terminal part of a shortened, mutated fragment of the lacZ gene (lacZ-MF) of vector pLZPWB1. As a result of the mutation, all methionine as well as cysteine residues are replaced by other amino-acid residues. In the fusion gene lacZ-MF-SAP of the resulting construct pSAP4 the two proteins are linked through a methionine residue. Expression of pSAP4 in E. coli W3110 in the presence of the inducer isopropylthiogalactoside (IPTG) led to production of fusion protein with a yield of approximately 50% of the total proteins synthesized. All SAP-immunoreactive fusion protein was found within the insoluble protein fraction and represented 40% of total proteins produced during expression. The fusion protein was subjected to cyanogen bromide cleavage. The overall yield of crude SAP with a purity of 80% was 10 mg/l of culture. The crude SAP was further purified by calmodulin-Sepharose affinity absorption. Characterisation by protein chemical analysis indicated the identity of recombinant SAP with authentic SAP purified from bull semen.

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Language(s): eng - English
 Dates: 1990-03
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 286449
ISI: A1990CW05400041
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Title: Biological Chemistry Hoppe-Seyler
  Alternative Title : Biol. Chem. Hoppe-Seyler
Source Genre: Journal
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Pages: - Volume / Issue: 371 (3) Sequence Number: - Start / End Page: 215 - 222 Identifier: ISSN: 0177-3593