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  RNA sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection.

Bauer, G. J. (1990). RNA sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection. Nucleic Acids Research, 18(4), 879-884.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0E38-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3633-5
Genre: Journal Article

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 Creators:
Bauer, G. J.1, Author              
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1Abteilung Biochemische Kinetik, MPI for biophysical chemistry, Max Planck Society, ou_578616              

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 Abstract: Although dideoxy terminated sequencing of RNA, using reverse transcriptase and oligodeoxynucleotide primers, is now a well established method, the accuracy is limited by sequence ambiguities due to unspecific chain termination events. A protocol is described which circumvents these ambiguities by using fluorescence labels tagged to dideoxynucleotides. Only chain terminations caused by dideoxynucleotides were detected while premature terminated cDNA's remain undetectable. In addition, the remaining multiple signals at nucleotide positions can be assigned to sequence heterogeneities within the RNA sequence to be determined.

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Language(s): eng - English
 Dates: 1990-02-25
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: eDoc: 286669
ISI: A1990CT25500025
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 18 (4) Sequence Number: - Start / End Page: 879 - 884 Identifier: ISSN: 0305-1048