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  The MIS12 complex is a protein interaction hub for outer kinetochore assembly.

Petrovic, A., Pasqualato, S., Dube, P., Krenn, V., Santaguida, S., Cittaro, D., et al. (2010). The MIS12 complex is a protein interaction hub for outer kinetochore assembly. Journal of Cell Biology, 190(5), 835-852. doi:10.1083/jcb.201002070.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-92F1-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-365B-6
Genre: Journal Article

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 Creators:
Petrovic, A., Author
Pasqualato, S., Author
Dube, P.1, Author              
Krenn, V., Author
Santaguida, S., Author
Cittaro, D., Author
Monzani, S., Author
Massimiliano, L., Author
Keller, J.2, Author              
Tarricone, A., Author
Maiolica, A., Author
Stark, H.1, Author              
Musaccio, A., Author
Affiliations:
1Research Group of 3D Electron Cryo-Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578577              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Kinetochores are nucleoprotein assemblies responsible for the attachment of chromosomes to spindle microtubules during mitosis. The KMN network, a crucial constituent of the outer kinetochore, creates an interface that connects microtubules to centromeric chromatin. The NDC80, MIS12, and KNL1 complexes form the core of the KMN network. We recently reported the structural organization of the human NDC80 complex. In this study, we extend our analysis to the human MIS12 complex and show that it has an elongated structure with a long axis of ∼22 nm. Through biochemical analysis, cross-linking–based methods, and negative-stain electron microscopy, we investigated the reciprocal organization of the subunits of the MIS12 complex and their contacts with the rest of the KMN network. A highlight of our findings is the identification of the NSL1 subunit as a scaffold supporting interactions of the MIS12 complex with the NDC80 and KNL1 complexes. Our analysis has important implications for understanding kinetochore organization in different organisms.

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Language(s): eng - English
 Dates: 2010-09-06
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: eDoc: 510657
DOI: 10.1083/jcb.201002070
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 190 (5) Sequence Number: - Start / End Page: 835 - 852 Identifier: -