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  Highly efficient single molecule detection in different micro and submicrometer channels with cw-excitation

Dorre, K., Stephan, J., & Eigen, M. (2001). Highly efficient single molecule detection in different micro and submicrometer channels with cw-excitation. Single Molecules, 2(3), 165-175.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-942F-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-F13E-6
Genre: Journal Article

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 Creators:
Dorre, K., Author
Stephan, J., Author
Eigen, M.1, Author              
Affiliations:
1Abteilung Biochemische Kinetik, MPI for biophysical chemistry, Max Planck Society, ou_578616              

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 Abstract: In this article we describe a detailed comparison between single molecule detection in three different types of microchannels. The overall detection efficiencies and the signal-to-noise ratios are compared. A new data evaluation technique that is used is explained in detail. The average number of photons per molecule is calculated from the measurements and the detection properties of each channel with respect to the diffusional characteristics of the dye molecules are investigated. From the detection efficiencies advantages and drawbacks of the individual methods are elucidated as well as possible applications of the techniques are illustrated.

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Language(s): eng - English
 Dates: 2001
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 17132
 Degree: -

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Title: Single Molecules
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 2 (3) Sequence Number: - Start / End Page: 165 - 175 Identifier: -