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  In‐vivo confocal scanning laser microscopy of the cerebral microcirculation

Dirnagl, U., Villringer, A., & Einhaeupl, K. M. (1992). In‐vivo confocal scanning laser microscopy of the cerebral microcirculation. Journal of Microscopy-Oxford, 165(1), 147-157. doi:10.1111/j.1365-2818.1992.tb04312.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-BFCA-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-D555-E
Genre: Journal Article

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Dirnagl, U., Author
Villringer, Arno1, Author              
Einhaeupl, K. M., Author
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1External Organizations, ou_persistent22              

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Free keywords: Blood cell velocity; Brain cortex; Cranial window; Fluorescence microscopy
 Abstract: Confocal scanning laser microscopy (CSLM) was used to study the microcirculation of the brain neocortex in anaesthetized rats. After removal of the dura mater, implantation of a closed cranial window, and intravenous injection of fluorescein, three‐dimensional reconstructions of cortical capillaries were performed down to a depth of 250 μm below the pial surface. Using a one‐dimensional approach (single line scanning), erythrocyte (negative contrast in fluorescently labelled plasma) and leucocyte (labelled with rhodamine 6 G) velocity and supply rate in cortical capillaries were measured. The effect of CO2‐inhalation on capillary blood flow dynamics was studied. Capillaries were imaged continuously for up to 1 h without changes in flow or fluorescence pattern. However, by increasing the laser power 10–100‐fold, aggregate formation was induced and capillaries were occluded, possibly due to damage to vascular endothelium. We conclude that CSLM can be used to study morphological and dynamic aspects of fluorescently labelled subsurface structures in organs of experimental animals. 1992 Blackwell Science Ltd

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 Dates: 1992
 Publication Status: Published in print
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 Identifiers: eDoc: 510918
Other: P9159
DOI: 10.1111/j.1365-2818.1992.tb04312.x
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Title: Journal of Microscopy-Oxford
Source Genre: Journal
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Pages: - Volume / Issue: 165 (1) Sequence Number: - Start / End Page: 147 - 157 Identifier: -