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  µPIXE for a µBrain: The vinegar fly’s brain, antenna, sensilla hairs and eye ion concentrations

Reinert, A., Barapatre, N., Sachse, S., & Reinert, T. (2011). µPIXE for a µBrain: The vinegar fly’s brain, antenna, sensilla hairs and eye ion concentrations. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 269(20), 2292-2296. doi:10.1016/j.nimb.2011.02.066.

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Reinert, Anja1, Author              
Barapatre, Nirav, Author
Sachse, Silke1, Author              
Reinert, Tilo, Author
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1BMBF Research Group Dr. S. Sachse, Olfactory Coding, Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421914              

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 Abstract: The vinegar fly Drosophila melanogaster is used as model organism to study a variety of different scientific purposes. Thus, our laboratory studies the olfactory system by neurobiological experiments. These techniques are often disruptive and need to compensate or exchange the body fluid, the lymph, with an artificial Ringer’s solution in defined compartments of the fly. The solution mainly contains Na, Cl, K and Ca and is to keep physiological conditions. Therefore, the knowledge about the ion concentrations in the respective Drosophila lymph is required for a correct mixture of the ions. This paper presents the spatially resolved concentrations of P, S, Cl, K, Ca, Fe, Cu and Zn in lyophilised head cryosections of Drosophila by using quantitative μPIXE at the ion beam facility LIPSION in Leipzig. The PIXE maps enable a detailed analysis of particular regions of interest down to a spatial resolution of 0.5 μm. We quantified the ion concentrations especially in the brain, the antenna and its sensilla hairs acting as the olfactory organ of the fly, in the compound eye and in the mouthparts. The averaged element concentrations of these main compartments are (in descending order): P: 90 mM, K: 81 mM, S: 38 mM, Cl: 18 mM, Ca: 4.9 mM, Fe: 1.4 mM, Zn: 1.2 mM, Cu: 0.06 mM. Certain structures or cavities possess a remarkably high concentration of particular elements and might reflect the different functions of the compartments. An example presented in more detail is the composition of the compound eye. Conclusively, our findings on the ion concentrations might be useful for the mixture of the Drosophila Ringer’s solution to ensure physiological conditions in experiments.

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 Dates: 20112011-03-012011
 Publication Status: Published in print
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 Identifiers: Other: SAC003
DOI: 10.1016/j.nimb.2011.02.066
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Title: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
  Abbreviation : NIM B
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier B.V.
Pages: - Volume / Issue: 269 (20) Sequence Number: - Start / End Page: 2292 - 2296 Identifier: ISSN: 0168-583X
CoNE: https://pure.mpg.de/cone/journals/resource/954925484704