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  A coiled coil trigger site is essential for rapid binding of synaptobrevin to the SNARE acceptor complex.

Wiederhold, K., Klöpper, T. H., Walter, A. M., Stein, A., Kienle, N., Sörensen, J. B., et al. (2010). A coiled coil trigger site is essential for rapid binding of synaptobrevin to the SNARE acceptor complex. Journal of Biological Chemistry, 285(28), 21549-21559. doi:10.1074/jbc.M110.105148.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0011-F048-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3392-1
Genre: Journal Article

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Wiederhold, K.1, Author              
Klöpper, T. H., Author
Walter, A. M.2, Author              
Stein, A.3, Author              
Kienle, N.1, Author              
Sörensen, J. B.2, Author              
Fasshauer, D.1, Author              
Affiliations:
1Research Group of Structural Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578596              
2Research Group of Molecular Mechanisms of the Exocytosis, MPI for biophysical chemistry, Max Planck Society, ou_578584              
3Research Group of Membrane Protein Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_2149675              

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 Abstract: Exocytosis from synaptic vesicles is driven by stepwise formation of a tight α-helical complex between the fusing membranes. The complex is composed of the three SNAREs: synaptobrevin 2, SNAP-25, and syntaxin 1a. An important step in complex formation is fast binding of vesicular synaptobrevin to the preformed syntaxin 1·SNAP-25 dimer. Exactly how this step relates to neurotransmitter release is not well understood. Here, we combined different approaches to gain insights into this reaction. Using computational methods, we identified a stretch in synaptobrevin 2 that may function as a coiled coil “trigger site.” This site is also present in many synaptobrevin homologs functioning in other trafficking steps. Point mutations in this stretch inhibited binding to the syntaxin 1·SNAP-25 dimer and slowed fusion of liposomes. Moreover, the point mutations severely inhibited secretion from chromaffin cells. Altogether, this demonstrates that the trigger site in synaptobrevin is crucial for productive SNARE zippering.

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Language(s): eng - English
 Dates: 2010-04-202010-07-09
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1074/jbc.M110.105148
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Title: Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Pages: - Volume / Issue: 285 (28) Sequence Number: - Start / End Page: 21549 - 21559 Identifier: ISSN: 0021-9258