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Journal Article

Arachidonic acid activates an open rectifier potassium channel in cultured rat cortical astrocytes


Schubert,  P.
Emeritus Group: Neuromorphology / Kreutzberg, MPI of Neurobiology, Max Planck Society;

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Ferroni, S., Valente, P., Caprini, M., Nobile, M., Schubert, P., & Rapisarda, C. (2003). Arachidonic acid activates an open rectifier potassium channel in cultured rat cortical astrocytes. Journal of Neuroscience Research, 72(3), 363-372. doi:10.1002/jnr.10580.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-2323-5
A pathophysiological increase in free arachidonic acid (AA) is thought to regulate the channel-mediated astrocytic swelling occurring in several brain injuries. We report that in cultured rat type-1 cortical astrocytes, exposure to 10 muM AA activates an open rectifier K+ channel, which exhibits many similarities with TREK/TRAAK members of the two-pore-domain K+ channel family KCNK. Patch-clamp experiments showed that the current developed with a long latency and was preceded by a depression of the previously described outward rectifier K+ conductance. Pharmacologic studies indicate that the K+ open rectifier was differentially sensitive to classic K+-channel blockers (quinine, quinidine, tetraethylammonium, and barium) and was inhibited potently by gadolinium ions. The activation of this K+ current occurred independently of the AA metabolism as pharmacologic inhibition of the lipoxygenase, cyclooxygenase, and cytochrome P450 epoxygenase signaling cascades did not alter the AA effect. Moreover, neither the neutralization of the NADPH-oxidase pathway nor scavenging intracellular free radicals modified the AA response. Finally, the AA-induced K+ current was unaffected by protein kinase C inhibitors. The activation mechanism of the K+ open rectifier was through an extracellular interaction of AA with the plasma membrane. RT-PCR analysis revealed that the AA-induced K+ conductance was mediated likely by TREK-2 channels. Collectively, the results demonstrate that in cultured cortical astrocytes, pathological levels of AA directly activate an open rectifier K+ channel, which may play a role in the control of K+ homeostasis under pathophysiological conditions. (C) 2003 Wiley-Liss, Inc.