English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Identification of neutrophil granule glycoproteins as Lewisx-containing ligands cleared by the scavenger receptor C-type lectin

MPS-Authors
/persons/resource/persons27732

Graham,  Sarah A.
Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, United Kingdom;
Language and Genetics Department, MPI for Psycholinguistics, Max Planck Society, Nijmegen, NL;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

SRCL paper-1.pdf
(Publisher version), 4MB

Supplementary Material (public)

graham_supp_info.pdf
(Supplementary material), 7MB

Citation

Graham, S. A., Antonopoulos, A., Hitchen, P. G., Haslam, S. M., Dell, A., Drickamer, K., et al. (2011). Identification of neutrophil granule glycoproteins as Lewisx-containing ligands cleared by the scavenger receptor C-type lectin. Journal of Biological Chemistry, 286, 24336-24349. doi:10.1074/jbc.M111.244772.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-28A9-6
Abstract
The scavenger receptor C-type lectin (SRCL) is a glycan-binding receptor that has the capacity to mediate endocytosis of glycoproteins carrying terminal Lewis(x) groups (Galβ1-4(Fucα1-3)GlcNAc). A screen for glycoprotein ligands for SRCL using affinity chromatography on immobilized SRCL followed by mass spectrometry-based proteomic analysis revealed that soluble glycoproteins from secondary granules of neutrophils, including lactoferrin and matrix metalloproteinases 8 and 9, are major ligands. Binding competition and surface plasmon resonance analysis showed affinities in the low micromolar range. Comparison of SRCL binding to neutrophil and milk lactoferrin indicates that the binding is dependent on cell-specific glycosylation in the neutrophils, as the milk form of the glycoprotein is a much poorer ligand. Binding to neutrophil glycoproteins is fucose dependent and mass spectrometry-based glycomic analysis of neutrophil and milk lactoferrin was used to establish a correlation between high affinity binding to SRCL and the presence of multiple, clustered terminal Lewis(x) groups on a heterogeneous mixture of branched glycans, some with poly N-acetyllactosamine extensions. The ability of SRCL to mediate uptake of neutrophil lactoferrin was confirmed using fibroblasts transfected with SRCL. The common presence of Lewis(x) groups in granule protein glycans can thus target granule proteins for clearance by SRCL. PCR and immunohistochemical analysis confirms that SRCL is widely expressed on endothelial cells and thus represents a distributed system which could scavenge released neutrophil glycoproteins both locally at sites of inflammation or systemically when they are released in the circulation.