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The Potato virus S resistance gene Ns maps to potato chromosome VIII

MPG-Autoren
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Gebhardt,  C.
Dept. of Plant Breeding and Genetics (Maarten Koornneef), MPI for Plant Breeding Research, Max Planck Society;
Dept. of Plant Breeding and Yield Physiology (Francesco Salamini), MPI for Plant Breeding Research, Max Planck Society;

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Zitation

Marczewski, W., Hennig, J., & Gebhardt, C. (2002). The Potato virus S resistance gene Ns maps to potato chromosome VIII. Theoretical and Applied Genetics, 105(4), 564-567.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0012-3D91-F
Zusammenfassung
The dominant allele Ns confers in potato resistance to Potato virus S (PVS). To identify the chromosomal location of Ns, we mapped the Ns-linked marker SCG1744, and the ISSR marker UBC811(600) to linkage group VIII of the RFLP map of a population that did not segregate for Ns. The map position of the Ns locus on chromosome VIII was confirmed with the detection of linkage between Ns and three RFLP markers, GP126, GP189 and CP16, known to be located in a corresponding region on potato chromosome VIII. PCR-based assays were developed for these RFLP markers. The PCR primers specific for GP 126 generated polymorphic products (STS marker). In the case of markers GP189 and CP16, informative polymorphism was revealed in the Ns population after digestion with the restriction enzymes HaeIII and HindIII, respectively. The genetic distance between Ns and the closest CP16 locus was 4.2 cM.