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Isolation and characterization of the Spindly homologue from tomato

MPG-Autoren
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Greb,  T.
Dept. of Plant Breeding and Yield Physiology (Francesco Salamini), MPI for Plant Breeding Research, Max Planck Society;

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Schmitz,  G.
Dept. of Plant Breeding and Genetics (Maarten Koornneef), MPI for Plant Breeding Research, Max Planck Society;

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Theres,  K.
Dept. of Plant Breeding and Genetics (Maarten Koornneef), MPI for Plant Breeding Research, Max Planck Society;

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Zitation

Greb, T., Schmitz, G., & Theres, K. (2002). Isolation and characterization of the Spindly homologue from tomato. Journal of Experimental Botany, 53(375), 1829-1830.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0012-3DB0-B
Zusammenfassung
The SPINDLY (SPY) gene is a crucial component of the gibberellin signal transduction pathway in Arabidopsis thaliana (L.) Heynh. In this study, the cloning of the SPINDLY- orthologous gene (LeSpy) from tomato (Lycopersicon esculentum Mill.) and its characterization are reported. SPY and LeSpy show high sequence similarity along their entire lengths, which is reflected in the conservation of exon-intron structure and of all sequence motives previously identified. To analyse the relationship between the Arabidopsis spindly and the tomato procera mutant, which show similar phenotypes, the LeSpy gene was characterized in wild-type and procera tomato plants. These analyses as well as mapping of LeSpy revealed that LeSpy and Procera are different genes.