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Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley

MPG-Autoren
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Cormack,  R. S.
Dept. of Biochemistry (Klaus Hahlbrock), MPI for Plant Breeding Research, Max Planck Society;

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Eulgem,  T.
Dept. of Biochemistry (Klaus Hahlbrock), MPI for Plant Breeding Research, Max Planck Society;

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Rushton,  P. J.
Dept. of Plant Microbe Interactions (Paul Schulze-Lefert), MPI for Plant Breeding Research, Max Planck Society;

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Koechner,  P.
Dept. of Plant Microbe Interactions (Paul Schulze-Lefert), MPI for Plant Breeding Research, Max Planck Society;

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Hahlbrock,  K.
Dept. of Biochemistry (Klaus Hahlbrock), MPI for Plant Breeding Research, Max Planck Society;

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Somssich,  I. E.
Dept. of Plant Microbe Interactions (Paul Schulze-Lefert), MPI for Plant Breeding Research, Max Planck Society;

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Zitation

Cormack, R. S., Eulgem, T., Rushton, P. J., Koechner, P., Hahlbrock, K., & Somssich, I. E. (2002). Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley. Biochimica et Biophysica Acta-Gene Structure and Expression, 1576(1-2), 92-100.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0012-3DC7-A
Zusammenfassung
Two new WRKY transcription factors from parsley (Petroselinum crispum), WRKY4 and WRKY5, were isolated using the yeast one- hybrid system. In yeast, both proteins interacted sequence- specifically with W boxes (TTGACC) and activated transcription. They appear to contain functional leucine zippers, which increase their affinities for W boxes. Co-transfection experiments in parsley protoplasts confirmed their in vivo- binding specificity for W boxes. Elicitor-mediated expression of the WRKY5 gene, the first parsley member of the group III family of WRKY proteins, is extremely transient, with high mRNA levels occurring within a time window of less than I h. WRKY4 and -5, as well as the previously identified parsley transcription factors WRKY 1 and -3, are encoded by immediate early elicitor-activated genes that differ in their sensitivity to cycloheximide (CHX) and their activation kinetics. We propose that a number of the pathways activated during the plant defense response require the induction of several distinct WRKY transcription factors with different DNA binding- site preferences to fine-tune the activation of a wide spectrum of target genes. (C) 2002 Elsevier Science B.V. All rights reserved.