Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Involvement of an NAD(P)H oxidase in the elicitor-inducible oxidative burst of soybean

There are no MPG-Authors in the publication available
External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Mithöfer, A., Daxberger, A., FromholdTreu, D., & Ebel, J. (1997). Involvement of an NAD(P)H oxidase in the elicitor-inducible oxidative burst of soybean. Phytochemistry, 45(6), 1101-1107. doi:10.1016/S0031-9422(97)00133-7.

Cite as: https://hdl.handle.net/11858/00-001M-0000-000F-4D12-1
The production of H2O2 in soybean (Glycine max L. cv Harosoy 63) suspension cells upon treatment with a 1,3-1,6-beta-glucan elicitor fraction from the cell walls of Phyrophthora sojae was investigated. The beta-glucan elicitor used in these studies was specifically recognized by the soybean beta-glucan-binding proteins and induced phytoalexin synthesis in the cell culture. Production of H2O2 was measured by the in situ oxidation of o-dianisidine and was both a time- and a concentration-dependent process, with a maximal response occurring after 20-25 min. Inhibitor studies showed that catalase, peroxidase inhibitors and ascorbate abolished the H2O2-mediated o-dianisidine oxidation. Diphenyleneiodonium, but not p-hydroxymercury benzoic acid, both known inhibitors of the mammalian NADPH oxidase involved in the oxidative burst of phagocytes, was a strong inhibitor of the inducible H2O2 production with a concentration for 50% inhibition of 4 mu M. The inhibitor studies also indicated that the oxidase might be at least in part responsible for H2O2 production by the cells. Furthermore, diphenyleneiodonium was the only inhibitor of O-2(-) synthesis in cell-free analyses with a concentration for 50% inhibition of 2.2 mu M. Neither ascorbate nor diphenyleneiodonium were able to inhibit elicitor-induced phytoalexin accumulation, indicating that in soybean H2O2 and O-2(-) may not be directly responsible for phytoalexin production, but may be an independent defence response. (C) 1997 Elsevier Science Ltd. All rights reserved.