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Book Chapter

Visualization of adult stem cells within their niches using the Drosophila germline as a model system.

MPS-Authors
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König,  A.
Research Group of Gene Expression and Signaling, MPI for biophysical chemistry, Max Planck Society;

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Shcherbata,  H. R.
Research Group of Gene Expression and Signaling, MPI for biophysical chemistry, Max Planck Society;

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1481524.pdf
(Publisher version), 286KB

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Citation

König, A., & Shcherbata, H. R. (2013). Visualization of adult stem cells within their niches using the Drosophila germline as a model system. In K. Turksen (Ed.), Stem Cell Niche: Methods and Protocols (pp. 25-33). New York: Springer; Humana Press.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-A753-0
Abstract
The germaria of the fruit fly Drosophila melanogaster present an excellent model to study germline stem cell–niche interactions. Two to three adult stem cells are surrounded by a number of somatic cells that form the niche. Here we describe how Drosophilae germaria can be dissected and specifically immuno-stained to allow for identification and analysis of both the adult stem cells and their somatic niche cells.