Combined experimental and computational approach to identify non-protein-coding 
RNAs in the deep-branching eukaryote Giardia intestinalis

Chen, Xiaowei Sylvia; Rozhdestvensky, Timofey S.; Collins, Lesley J.; Schmitz, Jurgen; Penny, David

doi:10.1093/nar/gkm474

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Combined experimental and computational approach to identify non-protein-coding RNAs in the deep-branching eukaryote Giardia intestinalis

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

http://nar.oxfordjournals.org/content/35/14/4619/suppl/DC1
(付録資料)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

NAR_ncRNA_Giardia_2007.pdf
(出版社版), 327KB

付随資料 (公開)

There is no public supplementary material available

引用

Chen, X. S., Rozhdestvensky, T. S., Collins, L. J., Schmitz, J., & Penny, D. (2007). Combined experimental and computational approach to identify non-protein-coding RNAs in the deep-branching eukaryote Giardia intestinalis. Nucleic Acids Research, 35, 4619-4628. doi:10.1093/nar/gkm474.

引用: https://hdl.handle.net/11858/00-001M-0000-000F-E5CA-2

要旨

Non-protein-coding RNAs represent a large proportion of transcribed sequences in eukaryotes. These RNAs often function in large RNA–protein complexes, which are catalysts in various RNA-processing pathways. As RNA processing has become an increasingly important area of research, numerous non-messenger RNAs have been uncovered in all the model eukaryotic organisms. However, knowledge on RNA processing in deep-branching eukaryotes is still limited. This study focuses on the identification of non-protein-coding RNAs from the diplomonad parasite Giardia intestinalis, showing that a combined experimental and computational search strategy is a fast method of screening reduced or compact genomes. The analysis of our Giardia cDNA library has uncovered 31 novel candidates, including C/D-box and H/ACA box snoRNAs, as well as an unusual transcript of RNase P, and double-stranded RNAs. Subsequent computational analysis has revealed additional putative C/D-box snoRNAs. Our results will lead towards a future understanding of RNA metabolism in the deep-branching eukaryote Giardia, as more ncRNAs are characterized.