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Journal Article

Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases.

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Höbartner,  C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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1539260.pdf
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1539260-Suppl.pdf
(Supplementary material), 625KB

Citation

Nawale, G. N., Gore, K. R., Höbartner, C., & Pradeepkumar, P. I. (2012). Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases. Chemical communications, 48(77), 9619-9621. doi:10.1039/c2cc35222b.


Cite as: https://hdl.handle.net/11858/00-001M-0000-000F-EB93-A
Abstract
The dual modified nucleotide 4′-C-aminomethyl-2′-O-methylthymidine 5′-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2′-O-methyl modification.