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False alleles derived from microbial DNA pose a potential source of error in microsatellite genotyping of DNA from faeces

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Bradley,  Brenda J.
Department of Primatology, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Vigilant,  Linda
Department of Primatology, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;
Molecular Genetics Laboratory, Department of Primatology, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Bradley, B. J., & Vigilant, L. (2002). False alleles derived from microbial DNA pose a potential source of error in microsatellite genotyping of DNA from faeces. Molecular Ecology Resources, 2(4), 602-605. doi:10.1046/j.1471-8286.2002.00302.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-0741-E
Abstract
Microsatellite genotyping of wild animals using DNA extracted from noninvasive samples such as faeces is a powerful means to identify individuals within a population and examine aspects of genetic social structure, such as relatedness and paternity. However, the use of the low quantities of poor quality DNA typically obtained from noninvasive samples can result in genotyping errors. Here we report the first instance of artefactural 'alleles' resulting from specific co-amplification of microorganismal DNA present in the total DNA derived from faeces.