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Journal Article

Tissue-specific distribution of secondary metabolites in rapeseed (Brassica napus)

MPS-Authors
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Fang,  Jingjing
Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

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Reichelt,  Michael
Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society;

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Hidalgo,  William
Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

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Agnolet,  Sara
Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society;

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Schneider,  Bernd
Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society;

External Resource

http://dx.doi.org/10.1371/journal.pone.0048006
(Publisher version)

Fulltext (restricted access)
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Fulltext (public)

NMR194.pdf
(Publisher version), 570KB

Supplementary Material (public)

NMR194s1.zip
(Supplementary material), 863KB

Citation

Fang, J., Reichelt, M., Hidalgo, W., Agnolet, S., & Schneider, B. (2012). Tissue-specific distribution of secondary metabolites in rapeseed (Brassica napus). PLoS One, 7(10): e48006. doi:10.1371/journal.pone.0048006.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-0CD1-B
Abstract
Four different parts, hypocotyl and radicle (HR), inner cotyledon (IC), outer cotyledon (OC), seed coat and endosperm (SE), were sampled from mature rapeseed (Brassica napus L.) by laser microdissection. Subsequently, major secondary metabolites, glucosinolates and sinapine, as well as three minor ones, a cyclic spermidine conjugate and two flavonoids, representing different compound categories, were qualified and quantified in dissected samples by high-performance liquid chromatography with diode array detection and mass spectrometry. No qualitative and quantitative difference of glucosinolates and sinapine was detected in embryo tissues (HR, IC and OC). On the other hand, the three minor compounds were observed to be distributed unevenly in different rapeseed tissues. The hypothetic biological functions of the distribution patterns of different secondary metabolites in rapeseed are discussed.