A new gene superfamily of pathogen-response (repat) genes in Lepidoptera: 
Classification and expression analysis

Navarro-Cerrillo, P.; Hernández-Martínez, P.; Vogel, Heiko; Ferré, J.; Herrero, S.

doi:10.1016/j.cbpb.2012.09.004

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

A new gene superfamily of pathogen-response (repat) genes in Lepidoptera: Classification and expression analysis

MPS-Authors

/persons/resource/persons4231

Vogel, Heiko
Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Navarro-Cerrillo, P., Hernández-Martínez, P., Vogel, H., Ferré, J., & Herrero, S. (2013). A new gene superfamily of pathogen-response (repat) genes in Lepidoptera: Classification and expression analysis. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 164(1), 10-17. doi:10.1016/j.cbpb.2012.09.004.

引用: https://hdl.handle.net/11858/00-001M-0000-0010-1142-A

要旨

Repat (REsponse to PAThogens) genes were first identified in the midgut of Spodoptera exigua (Lepidoptera:
Noctuidae) in response to Bacillus thuringiensis and baculovirus exposure. Since then, additional repat gene
homologs have been identified in different studies. In this study the comprehensive larval transcriptome
from S. exigua was analyzed for the presence of novel repat-homolog sequences. These analyses revealed
the presence of at least 46 repat genes in S. exigua, establishing a new gene superfamily in this species.
Phylogenetic analysis and studies of conserved motifs in these hypothetical proteins have allowed their
classification in two main classes, αREPAT and βREPAT. Studies on the transcriptional response of repat
genes have shown that αREPAT and βREPAT differ in their sequence but also in the pattern of regulation.
The αREPAT were mainly regulated in response to the Cry1Ca toxin from B. thuringiensis but not to the
increase in the midgut microbiota load. In contrast, βREPAT were neither responding to Cry1Ca toxin nor
to midgut microbiota. Differential expression between midgut stem cells and the whole midgut tissue was
studied for the different repat genes revealing changes in the gene expression distribution between midgut
stem cells and midgut tissue in response to midgut microbiota. This high diversity found in their sequence
and in their expression profile suggests that REPAT proteins may be involved in multiple processes thatcould be of relevance for the understanding of the insect gut physiology.