Identification of teleost Thy-1 and association with the microdornain/lipid 
raft reggie proteins in regenerating CNS axons

Deininger, S. O.; Rajendran, L.; Lottspeich, F.; Przybylski, M.; Illges, H.; Stuermer, C. A. O.; Reuter, A.

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Identification of teleost Thy-1 and association with the microdornain/lipid raft reggie proteins in regenerating CNS axons

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Lottspeich, F.
Lottspeich, Friedrich / Protein Analysis, Max Planck Institute of Biochemistry, Max Planck Society;

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Deininger, S. O., Rajendran, L., Lottspeich, F., Przybylski, M., Illges, H., Stuermer, C. A. O., et al. (2003). Identification of teleost Thy-1 and association with the microdornain/lipid raft reggie proteins in regenerating CNS axons. Molecular and Cellular Neuroscience, 22(4), 544-554.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-6C3D-9

Abstract

During regeneration, retinal ganglion cell axons in fish upregulate a cell surface protein that is recognized by the monoclonal antibody (mAB) M802. M802 antigen appeared to be linked to the intracellular, membrane-associated lipid raft/microdomain proteins reggie-1 and reggie-2 that were previously shown to be reexpressed in axon-regenerating neurons [Development 124 (1997), 577]. Here, we report the isolation of the M802 antigen and its identification as the teleost homolog of mammalian Thy-1. Fish Thy-1 is detected in the same detergent-in soluble lipid raft fractions from a fibroblast cell line and from axon regenerating retinae as reggie-1 and 2. Importantly, mAB M802 coimmunoprecipitates reggie-1 and 2 from this lipid raft fraction, implying that fish Thy-l and reggies interact. This correlates with their colocalization in growing cell processes after M802 antigen/Thy-1 activation with mAB M802. These findings suggest a role of clustered M802 antigen/Thy-1 in reggie raft microdomains for cell growth and axon regeneration. (C) 2003 Elsevier Science (USA). All rights reserved.