Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect

MPG-Autoren
/persons/resource/persons78269

Krempelhuber,  A. von
Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Schreiner, T., Mohrs, M. R., Blau-Wasser, R., Krempelhuber, A. v., Steinert, M., Schleicher, M., et al. (2002). Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect. Eukaryotic Cell, 1(6), 906-914.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-6DC8-5
Zusammenfassung
Comitin is an F-actin binding and membrane-associated protein from Dictyostelium discoideum, which is present on Golgi and vesicle membranes and changes its localization in response to agents affecting the cytoskeleton. To investigate its in vivo functions we have generated knockout mutants by gene replacement. Based on comitin's in vitro functions we examined properties related to vesicular transport and microfilament function. Whereas cell growth, pinocytosis, secretion, chemotaxis, motility, and development were unaltered, comitin- lacking cells were impaired in the early steps of phagocytosis of Saccharomyces cerevisiae particles and of Escherichia coli, whereas uptake of latex beads was unaffected. Furthermore, the lack of comitin positively affected survival of pathogenic bacteria. Mutant cells also showed an altered response to hyperosmotic shock in comparison to the wild type. The redistribution of comitin during hyperosmotic shock in wild- type cells and its presence on early phagosomes suggest a direct involvement of comitin in these processes.