Chromosome-induced microtubule assembly mediated by TPX2 is required for 
spindle formation in HeLa cells

Gruss, O. J.; Wittmann, M.; Yokoyama, H.; Pepperkok, R.; Kufer, T.; Sillje, H.; Karsenti, E.; Mattaj, I. W.; Vernos, I.

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells

MPG-Autoren

/persons/resource/persons78280

Kufer, T.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78710

Sillje, H.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

Gruss, O. J., Wittmann, M., Yokoyama, H., Pepperkok, R., Kufer, T., Sillje, H., et al. (2002). Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nature Cell Biology, 4(11), 871-879.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0010-6DFC-4

Zusammenfassung

In Xenopus laevis egg extracts, TPX2 is required for the Ran- GTP-dependent assembly of microtubules around chromosomes. Here we show that interfering with the function of the human homologue of TPX2 in HeLa cells causes defects in microtubule organization during mitosis. Suppressing the expression of human TPX2 by RNA interference leads to the formation of two microtubule asters that do not interact and do not form a spindle. Our results suggest that in vivo, even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells.