Arrangement of subunits in 20 s particles consisting of nsf, snaps, and snare 
complexes

Hohl, T. M.; Parlati, F.; Wimmer, C.; Rothman, J. E.; Söllner, T. H.; Engelhardt, H.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Arrangement of subunits in 20 s particles consisting of nsf, snaps, and snare complexes

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Hohl, T. M., Parlati, F., Wimmer, C., Rothman, J. E., Söllner, T. H., & Engelhardt, H. (1998). Arrangement of subunits in 20 s particles consisting of nsf, snaps, and snare complexes. Molecular Cell, 2(5), 539-548.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-71E7-B

Abstract

The structure of 20 S particles, consisting of NSF, SNAPs, and SNARE complexes, was analyzed by electron microscopy and fluorescence resonance energy transfer. Structural changes associated with the binding of alpha-SNAP and NSF to SNARE complexes define the contribution of each component to the 20 S particle structure. The synaptic SNARE complex forms a 2.5 x 15 nm rod, alpha-SNAP binds laterally to the rod, increasing its width but not its length. NSF binds to one end of the SNAP/SNARE complex; the resulting 20 S particles measure 22 nm in length and vary in width from 6 nm at their narrowest point to 13.5 nm at their widest. The transmembrane domains of VAMP and syntaxin emerge together at the NSF-distal end of 20 S particles, adjacent to the amino terminus of alpha-SNAP. [References: 61]