Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben
Zeitschriftenartikel

Orientation and two-dimensional organization of proteins at chelator lipid interfaces

MPG-Autoren
Es sind keine MPG-Autoren in der Publikation vorhanden
Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Dorn, I. T., Pawlitschko, K., Pettinger, S. C., & Tampe, R. (1998). Orientation and two-dimensional organization of proteins at chelator lipid interfaces. Biological Chemistry, 379(8-9), 1151-1159.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0010-724A-7
Zusammenfassung
The analysis how proteins interact or assemble with each other in time and space is of central interest. Bio-functionalized interfaces can be applied to study protein-protein interactions in solution or elementary biological processes at membranes. Chelator lipid layers ave well suited for these applications as they specifically bind histidine-tagged fusion proteins and further mimic the two-dimensional world of biological membranes. Here, we used green fluorescent protein (GFP) as a model to study its reversible, functional, and oriented immobilization via histidine-tag at chelator lipid interfaces by various surface sensitive techniques. Taking advantage of the self-organizing properties of chelator lipids, the association and dissociation kinetics, the surface density as well as the organization of the protein in two-dimensional arrays can be controlled. The chelator lipid system can be used for bioanalytical and structural studies as well as to examine recognition processes at membranes. [References: 46]