Electron tomography of single ice-embedded macromolecules - three-dimensional 
alignment and classification

Walz, J.; Typke, D.; Nitsch, M.; Koster, A. J.; Hegerl, R.; Baumeister, W.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Walz, J., Typke, D., Nitsch, M., Koster, A. J., Hegerl, R., & Baumeister, W. (1997). Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification. Journal of Structural Biology, 120(3), 387-395.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-726E-8

Abstract

From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. (C) 1997 Academic Press. [References: 24]