Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Three-dimensional structure of the regular surface layer (HPI layer) of Deinococcus radiodurans

There are no MPG-Authors available
External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Baumeister, W., Barth, M., Hegerl, R., Guckenberger, R., Hahn, M., & Saxton, W. O. (1986). Three-dimensional structure of the regular surface layer (HPI layer) of Deinococcus radiodurans. Journal of Molecular Biology., 187(2), 241-250.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-74FA-B
The low-resolution structure of the regular surface layer of Deinococcus radiodurans has been determined from negatively stained specimens by three-dimensional electron microscopy. The layer has P6 symmetry, a lattice constant of 18 nm and a thickness of 6.5 nm. Three-dimensional reconstruction was performed by a hybrid real space/Fourier space approach that incorporates partial compensation of lattice distortions: The model obtained is discussed in the light of independent information about the surface structure of this layer, derived from metal shadowing and surface relief reconstruction. While agreement is quite satisfactory for the apparently more rigid inner surface, the outer surface shows severe flattening effects. The structure of the HPI layer is compared with other bacterial surface layers using a classification scheme that is outlined in the Appendix.