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Entwicklung einer array-basierten Karte für das Zuckerrübengenom


Mittermayr,  Lukas
Max Planck Society;

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Mittermayr, L. (2009). Entwicklung einer array-basierten Karte für das Zuckerrübengenom. Master Thesis, Universität Salzburg, Salzburg.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7E13-0
Abstract The sugarbeet Beta vulgaris var. vulgaris is a biannual plant that belongs to the Amaranthaceae family with a genome size of 758Mb and a diploid chromosome set of 18 chromosomes. Almost all of the consumed sugar in Europe derives from the sugar of sugarbeets, which are cultivated in Europe. This is one reason why it is important to have dense genetic maps of the sugarbeets’ genome. Another reason is the breeding of resistance sugarbeet lines. The point of this project was to find new genetic markers for the sugarbeet genome with a new array-based method, which could connect a genetic with a physical map. To detect polymorphisms between two parental sugarbeet lines (P1 and P2) amplicons were made from their genomic DNA. The amplicons were fluorescence marked and got hybridized on a Microarray. The Oligonukleotide of these array derived from sugarbeet BAC-endsequences and ESTs, which are also used in a physical mapping project. The custom-made Microarrays were ordered by Agilent Technologies. The oligonucleotides which detect a polymorphism, almost 14500 of 45200 tested, were set up to a new Microarray. On this Microarray 196 genotypes of the F2-generation of the parental lines (P1 and P2) had been hybridized in order to calculate a genetic map with this data. The program Mapmaker 3.0 calculated 293 of the 14500 marker-candidates into a known genetic map with already more than 715 known markers.