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Fast biosynthesis of GFP molecules : a single-molecule fluorescence study

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Nierhaus,  Knud H.
Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society;

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Citation

Katranidis, A., Atta, D., Schlesinger, R., Nierhaus, K. H., Choli-Papadopoulou, T., Gregor, I., et al. (2009). Fast biosynthesis of GFP molecules: a single-molecule fluorescence study. Angewandte Chemie International Edition, 48(10), 1758-1761. doi:10.1002/anie.200806070.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-7E57-7
Abstract
It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute.