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Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome

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Stelzl,  Ulrich
Molecular Interaction Networks (Ulrich Stelzl), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Pech,  Markus
Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Nierhaus,  Knud H.
Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society;

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Citation

Bishop, A. Ö. T., Stelzl, U., Pech, M., & Nierhaus, K. H. (2008). Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome. In R.-J. Lin (Ed.), RNA-Protein Interaction Protocols (pp. 129-151). Berlin [et al]: Springer.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7EF0-D
Abstract
Analogs of naturally occurring substances obtained by chemical modifications are powerful tools to study intra- and intermolecular interactions. We have used the phosphorothioate technique to analyze RNA-protein interactions, here the interactions of transfer RNAs (tRNAs) with the three ribosomal binding sites. We describe preparation and purification of thioated tRNAs, formation of functional complexes of programmed ribosomes with tRNAs, and the evaluation of the observed phosphorothioate footprints on the tRNAs.