A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of 
the Human Transcriptome

Sultan, Marc; Schulz, Marcel H.; Hugues, Richard; Magen, Alon; Klingenhoff, Andreas; Scherf, Matthias; Seifert, Martin; Borodina, Tatjana; Soldatov, Aleksey; i Parkhomchuk, Dmitr; Schmidt, Dominic; O'Keeffe, Sean; Haas, Stefan; Vingron, Martin; Lehrach, Hans; Yaspo, Marie-Laure

doi:10.1126/science.1160342

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A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome

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Sultan, Marc
Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Schulz, Marcel H.
Max Planck Society;

Hugues, Richard
Max Planck Society;

Magen, Alon
Max Planck Society;

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Borodina, Tatjana
Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50561

Soldatov, Aleksey
Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

i Parkhomchuk, Dmitr
Max Planck Society;

Schmidt, Dominic
Max Planck Society;

O'Keeffe, Sean
Max Planck Society;

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Haas, Stefan
Gene Structure and Array Design (Stefan Haas), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50613

Vingron, Martin
Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50409

Lehrach, Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50655

Yaspo, Marie-Laure
Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Citation

Sultan, M., Schulz, M. H., Hugues, R., Magen, A., Klingenhoff, A., Scherf, M., et al. (2008). A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome. Science, 321(5891), 956-960. doi:10.1126/science.1160342.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7F47-3

Abstract

The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and 34% to nonannotated genomic regions. On the basis of known transcripts, RNA-Seq can detect 25% more genes than can microarrays. A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing.