Characterization of Tau in cerebrospinal fluid using mass spectrometry

Portelius, Erik; Hansson, Sara F.; Tran, Ai Jun; Zetterberg, Henrik; Grognet, Pierre; Vanmechelen, Eugeen; Höglund, Kina; Brinkmalm, Gunnar; Westman-Brinkmalm, Ann; Nordhoff, Eckhard; Blennow, Kaj; Gobom, Johan

doi:10.1021/pr7008669

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Characterization of Tau in cerebrospinal fluid using mass spectrometry

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Nordhoff, Eckhard
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Gobom, Johan
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Citation

Portelius, E., Hansson, S. F., Tran, A. J., Zetterberg, H., Grognet, P., Vanmechelen, E., et al. (2008). Characterization of Tau in cerebrospinal fluid using mass spectrometry. Journal of Proteome Reserach, 7(2), 2114-2120. doi:10.1021/pr7008669.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7FF4-E

Abstract

The neurodegenerative disorder Alzheimer’s disease (AD) is the most common cause of dementia in the elderly. The presence of neurofibrillary tangles, consisting of hyperphosphorylated tau protein, is one of the major neuropathologic characteristics of the disease, making this protein an attractive biomarker for AD and a possible target for therapy. Here, we describe an optimized immunoprecipitation mass spectrometry method that enables, for the first time, detailed characterization of tau in human cerebrospinal fluid. The identities of putative tau fragments were confirmed using nanoflow liquid chromatography and tandem mass spectrometry. Nineteen tryptic fragments of tau were detected, of which 16 are found in all tau isoforms while 3 represented unique tau isoforms. These results pave the way for clinical CSF studies on the tauopathies.