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Journal Article

Functional genomics using high-throughput RNA interference


Vanhecke,  Dominique
Max Planck Society;


Janitz,  Michal
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Vanhecke, D., & Janitz, M. (2005). Functional genomics using high-throughput RNA interference. Drug Discovery Today, 10(3), 205-212. doi:10.1016/S1359-6446(04)03352-5.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-86E7-8
RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells. The review provides a comprehensive overview on current RNA interference-based high throughput genomic technologies. The cell array- and microwell plate-based loss-of-function cell assays are presented.