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Journal Article

Proteomic approaches in plant biology


Kersten,  Birgit
Max Planck Society;


Feilner,  Tanja
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;


Angenendt,  Philipp
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Giavalisco,  Patrick
Max Planck Society;

Brenner,  Wolfram
Max Planck Society;

Bürkle,  Lukas
Max Planck Society;

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Kersten, B., Feilner, T., Angenendt, P., Giavalisco, P., Brenner, W., & Bürkle, L. (2004). Proteomic approaches in plant biology. Current Proteomics, 1(2), 131-144.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-8934-4
Proteomic approaches play an important role in the study of complex biological systems. The application of proteomic technologies in plant science has been strongly supported by the completion of genome sequence projects of the model plants Arabidopsis thaliana and rice. This review focuses on the state of proteomic technologies with special emphasis on their application in plant biology. An overview of recent developments in 2-dimensional gel electrophoresis and liquid chromatography-based multidimensional protein identification technology, MudPIT is provided. These techniques are commonly combined with mass spectrometric methods for identification of proteins. Furthermore, protein expression profiling by antibody arrays and the selection of required recombinant antibodies by phage display are described. Interaction studies, using functional protein microarrays or the yeast two-hybrid system are presented as powerful techniques to gain insights into the function of proteins. Advantages and limitations of the described methods as well as their current and potential future applications in plant research are discussed.