English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Identification of downstream targets of the nodal signaling pathway.

MPS-Authors

Aanstad,  Pia
Max Planck Society;

/persons/resource/persons50409

Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Bennett, J., Joubin, K., Cheng, S., Aanstad, P., Lehrach, H., & Schier, A. F. (2003). Identification of downstream targets of the nodal signaling pathway. Developmental Biology, 259(2), 527-527.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-89ED-6
Abstract
Nodal signaling is critical for a variety of processes during vertebrate development, including gastrulation and left—right patterning. Although many of the molecules that play a role in the transduction of Nodal signals are known, the current list of genes known to be regulated by Nodal signals is incomplete. We identified putative Nodal regulated genes by differential RNA hybridization against a zebrafish cDNA library of about 14,000 cDNAs. Putative Nodal-regulated genes were also identified by screening zebrafish gene expression databases for genes expressed in the margin or shield of early gastrula-stage embryos. Putative Nodal-regulated genes were then screened by in situ hybridization against wild-type and maternal-zygotic one eyed pinhead embryos, which lack all Nodal signaling. This led to the identification of 57 unique Nodal-regulated genes. We are currently analyzing the function and regulation of these genes.