Extraction of proteins from plant tissues for two-dimensional electrophoresis 
analysis

Giavalisco, Patrick; Nordhoff, Eckhard; Lehrach, Hans; Gobom, Joerg; Klose, Joachim

doi:10.1002/elps.200390016

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis

MPS-Authors

Giavalisco, Patrick
Max Planck Society;

/persons/resource/persons50448

Nordhoff, Eckhard
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50409

Lehrach, Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Gobom, Joerg
Max Planck Society;

Klose, Joachim
Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Giavalisco, P., Nordhoff, E., Lehrach, H., Gobom, J., & Klose, J. (2003). Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis. Special Issue HPCE 2003, 207-216. doi:10.1002/elps.200390016.

引用: https://hdl.handle.net/11858/00-001M-0000-0010-8AF9-2

要旨

To increase the number of proteins detectable by two-dimensional electrophoresis (2-DE) in plants, we present a new procedure for extracting total proteins from plant tissue. This method avoids any loss of proteins in the course of sample preparation and results in two different fractions, one comprising mainly the cytoplasmatic proteins, the other one containing predominantly structure bond proteins. 2-DE patterns obtained from these two fractions show that the total number of different protein spots detected exceeds the degree of resolution commonly reported for plant proteins threefold.