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How does tmRNA move through the ribosome?


Ivanov,  Pavel V.
Max Planck Society;

Zvereva,  Maria I.
Max Planck Society;

Teraoka,  Yoshika
Max Planck Society;


Nierhaus,  Knud H.
Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society;

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Ivanov, P. V., Zvereva, M. I., Shpanchenko, O. V., Dontsova, O. A., Bogdanov, A. A., Aglyamova, G. V., et al. (2002). How does tmRNA move through the ribosome? FEBS Letters, 514(1), 55-59.

To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon–anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.