Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Quantitative intensity-based FRET approaches-A comparative snapshot.

MPG-Autoren
/persons/resource/persons59532

Woehler,  A.
Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15570

Neher,  E.
Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society;

Volltexte (frei zugänglich)

1586520.pdf
(Verlagsversion), 184KB

Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Zeug, A., Woehler, A., Neher, E., & Ponimaskin, E. G. (2012). Quantitative intensity-based FRET approaches-A comparative snapshot. Biophysical Journal, 103(9), 1821-1827. doi:10.1016/j.bpj.2012.09.031.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8D1D-3
Zusammenfassung
Förster resonance energy transfer (FRET) has become an important tool for analyzing different aspects of interactions among biological macromolecules in their native environments. FRET analysis has also been successfully applied to study the spatiotemporal regulation of various cellular processes using genetically encoded FRET-based biosensors. A variety of procedures have been described for measuring FRET efficiency or the relative abundance of donor-acceptor complexes, based on analysis of the donor fluorescence lifetime or the spectrally resolved fluorescence intensity. The latter methods are preferable if one wants to not only quantify the apparent FRET efficiencies but also calculate donor-acceptor stoichiometry and observe fast dynamic changes in the interactions among donor and acceptor molecules in live cells. This review focuses on a comparison of the available intensity-based approaches used to measure FRET. We discuss their strengths and weaknesses in terms of FRET quantification, and provide several examples of biological applications.