English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

mMaple: A photoconvertible fluorescent protein for use in multiple imaging modalities.

MPS-Authors
/persons/resource/persons36457

Bates,  M.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://www.plosone.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0051314&representation=PDF
(Publisher version)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

1587744.pdf
(Publisher version), 2MB

Supplementary Material (public)

1587744_Suppl_1.tif
(Supplementary material), 303KB

1587744_Suppl_2.tif
(Supplementary material), 402KB

1587744_Suppl_3.tif
(Supplementary material), 6MB

1587744_Suppl_4.tif
(Supplementary material), 462KB

1587744_Suppl_5.tif
(Supplementary material), 2MB

1587744_Suppl_6.tif
(Supplementary material), 2MB

1587744_Suppl_7.tif
(Supplementary material), 2MB

1587744_Suppl_8.tif
(Supplementary material), 206KB

1587744_Suppl_9.tif
(Supplementary material), 716KB

1587744_Suppl_10.tif
(Supplementary material), 2MB

1587744_Suppl_11.tif
(Supplementary material), 3MB

1587744_Suppl_12.mov
(Supplementary material), 256KB

1587744_Suppl_13.mov
(Supplementary material), 145KB

1587744_Suppl_14.doc
(Supplementary material), 35KB

Citation

McEvoy, A. L., Hoi, H., Bates, M., Platonova, E., Cranfill, P. J., Baird, M. W., et al. (2012). mMaple: A photoconvertible fluorescent protein for use in multiple imaging modalities. PLoS One, 7(12): e51314. doi:10.1371/journal.pone.0051314.


Cite as: https://hdl.handle.net/11858/00-001M-0000-000E-72D2-C
Abstract
Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFPcontaining construct.