Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions

Scheibe, Marion; Butter, Falk; Hafner, Markus; Tuschl, Thomas; Mann, Matthias

doi:10.1093/nar/gks746

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions

MPS-Authors

/persons/resource/persons78974

Scheibe, Marion
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77831

Butter, Falk
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons15941

Tuschl, Thomas
Research Group of Combinatorical Biochemistry, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons78356

Mann, Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

9897.full.pdf
(全文テキスト（全般）), 463KB

付随資料 (公開)

There is no public supplementary material available

引用

Scheibe, M., Butter, F., Hafner, M., Tuschl, T., & Mann, M. (2012). Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions. NUCLEIC ACIDS RESEARCH, 40(19), 9897-9902. doi:10.1093/nar/gks746.

引用: https://hdl.handle.net/11858/00-001M-0000-000E-776B-1

要旨

Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs identify a group of proteins bound to an RNA. We investigated binding sites for five different proteins (IGF2BP1-3, QKI and PUM2) exhibiting different binding patterns. We report near perfect agreement between the two approaches. Nevertheless, they are non-redundant, and ideally complement each other to map the RNA-protein interaction network.