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Journal Article

Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

MPS-Authors
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Tkach,  I.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Höbartner,  C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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Wachowius,  F.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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Sicoli,  G.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Bennati,  M.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

External Ressource
Fulltext (public)

1629145.pdf
(Publisher version), 3MB

Supplementary Material (public)

1629145_Supplement_1.pdf
(Supplementary material), 3MB

Citation

Tkach, I., Pornsuwan, S., Höbartner, C., Wachowius, F., Sigurdsson, S., Baranova, T. Y., et al. (2013). Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation. Physical Chemistry Chemical Physics, 15(10), 3433-3437. doi:10.1039/c3cp44415e.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-801F-F
Abstract
Pulsed electron–electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.