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Journal Article

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.

MPS-Authors
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Honigmann,  A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

Müller,  V.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15024

Eggeling,  C.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

Fulltext (public)

1684353.pdf
(Publisher version), 7MB

Supplementary Material (public)

1684353-Suppl-1.pdf
(Supplementary material), 3MB

1684353-Suppl-Video1.avi
(Supplementary material), 13MB

1684353-Suppl-Video2.avi
(Supplementary material), 837KB

Citation

Lukinavičius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., et al. (2013). A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature chemistry, 5(2), 132-139. doi:10.1038/nchem.1546.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-BB40-E
Abstract
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon–rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.