Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Using radiocarbon to determine the mycorrhizal status of fungi


Hobbie,  E. A.
Department Biogeochemical Systems, Prof. D. Schimel, Max Planck Institute for Biogeochemistry, Max Planck Society;


Van Klinken,  G. J.
Service Facility 14C Lab, Dr. Geert van Klinken, Max Planck Institute for Biogeochemistry, Max Planck Society;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Hobbie, E. A., Weber, N. S., Trappe, J. M., & Van Klinken, G. J. (2002). Using radiocarbon to determine the mycorrhizal status of fungi. New Phytologist, 156(1), 129-136. doi:10.1046/j.1469-8137.2002.00496.x.

Cite as: https://hdl.handle.net/11858/00-001M-0000-000E-CF07-2
Measurements of C-13 in fungal sporocarps are useful in assessing mycorrhizal or saprotrophic status. Because C-14 measurements can indicate the age of fungal carbon (C) and mycorrhizal fungi depend closely on recent photosynthate, C-14 may provide additional insight into possible mycorrhizal status. Sporocarps, needles, and litter from Woods Creek, OR, USA together with archived sporocarps were measured for C-14 content by accelerator mass spectrometry. Known mycorrhizal fungi resembled current-year needles (Amanita, Cantharellus and Gomphidius) or atmospheric CO2 (Tuber) in C-14 and indicated an average age of 0-2 yr for incorporated C, whereas saprotrophic genera (Pleurocybella , Lepiota and Hypholoma) were composed of C at least 10 yr old. Of genera tentatively considered mycorrhizal from previous work with C-13, only Otidia and Sowerbyella appeared mycorrhizal from C-14 measurements, whereas Aleuria, Clavulina, Paurocotylis and Ramaria (sensu lato) consisted of older carbon and were presumably saprotrophic. C-14 clearly separated known mycorrhizal or saprotrophic fungi and indicated C-13 measurements should be interpreted cautiously on species of unknown status. C-14 results for needles and mycorrhizal fungi suggested that C sources other than atmospheric CO2 may contribute small amounts of C. Possible sources include storage of carbohydrates and amino acids, organic nitrogen uptake, and incorporation of soil-respired CO2 by anaplerotic or photosynthetic pathways.