Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative 
translational initiation.

Chua, J. J.; Schob, C.; Rehbein, M.; Gkogkas, C. G.; Richter, D.; Kindler, S.

doi:10.1038/srep00484

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Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative translational initiation.

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Chua, J. J.
Research Group of Protein Trafficking in Synaptic Development and Function, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Chua, J. J., Schob, C., Rehbein, M., Gkogkas, C. G., Richter, D., & Kindler, S. (2012). Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative translational initiation. Scientific Reports, 2: 484. doi:10.1038/srep00484.

Cite as: https://hdl.handle.net/11858/00-001M-0000-000E-E8BD-A

Abstract

In mammalian neurons, targeting and translation of specific mRNAs in dendrites contribute to synaptic plasticity. After nuclear export, mRNAs designated for dendritic transport are generally assumed to be translationally dormant and activity of individual synapses may locally trigger their extrasomatic translation. We show that the long, GC-rich 59-untranslated region of dendritic SAPAP3 mRNA restricts translation initiation via a mechanism that involves an upstream open reading frame (uORF). In addition, the uORF enables the use of an alternative translation start site, permitting synthesis of two SAPAP3 isoforms from a single mRNA. While both isoforms progressively accumulate at postsynaptic densities during early rat brain development, their levels relative to each other vary in different adult rat brain areas. Thus, alternative translation initiation events appear to regulate relative expression of distinct SAPAP3 isoforms in different brain regions, which may function to influence synaptic plasticity.