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Micro-optical lens array for fluorescence detection in droplet-based microfluidics.

MPG-Autoren
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Lim,  Jiseok
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Konrad,  M.
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Zitation

Lim, J., Gruner, P., Konrad, M., & Baret, J. C. (2013). Micro-optical lens array for fluorescence detection in droplet-based microfluidics. Lab on a Chip, 13(8), 1472-1475. doi:10.1039/C3LC41329B.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0013-7643-6
Zusammenfassung
We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase in the fluorescence signal and in improved spatial resolution. Using an array of microlenses, massively parallel detection of droplets containing fluorescent dyes was achieved, leading to detection throughputs of about 2000 droplets per second and per lens, parallelized over 625 measurement points.