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Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture

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Ritter,  J. B.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Genzel,  Y.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Reichl,  U.
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Citation

Ritter, J. B., Genzel, Y., & Reichl, U. (2007). Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture. In R. Smith (Ed.), Cell Technology for Cell Products: Proceedings of the 19th ESACT Meeting (pp. 603-605). Dordrecht, The Netherlands: Springer.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0013-98B0-9
Abstract
Some intracellular intermediate metabolites can also be found in the medium supernatant at micromolar concentrations. In this work, we are investigating extracellular concentrations of five organic acids (succinic, malic, fumaric, citric and isocitric acid) during cell growth and after viral infection of MDCK cells.