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Journal Article

Preparative Size-Exclusion Chromatography

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Kalbfuss,  B.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Reichl,  U.
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Citation

Kalbfuss, B., & Reichl, U. (2006). Preparative Size-Exclusion Chromatography. GIT Laboratory Journal Europe, 10(4), 18-20.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0013-9A05-E
Abstract
Size-exclusion chromatography in group separation mode is an efficient unit operation for the separation of influenza virions from host cell protein [1]. In order to avoid this operation becoming a bottleneck in vaccine production processes, however, the column load has to be optimised balancing productivity with purity. Design principles and the results of a loading study on Sepharose 4 FF are resented in this article.