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Journal Article

Simultaneous Determination of Five Bioactive Flavonoids in Hypericum japonicum Thunb by High-performance Liquid Chromatography

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Citation

Li, J., Jiang, B., Liu, X., Zhang, J., Chen, H., & Bi, S. (2007). Simultaneous Determination of Five Bioactive Flavonoids in Hypericum japonicum Thunb by High-performance Liquid Chromatography. Asian Journal of Traditional Medicines, 2(2), 75-81.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-CEAD-2
Abstract
A simple reversed-phase liquid chromatographic method has been established for the simultaneous quantification of five bioactive flavonoids, i.e., taxifolin-7-O-rhamnoside, isoquercitrin, quercitrin, quercetin and kaempferol in Hypericum japonicum Thunb. Chromatographic separations were achieved on a Luna C18 column (250 mm×4.6 mm, 5 amp;956;m) with a gradient of methanol and 0.5 aqueous acetic acid (v/v) as mobile phase and UV detection at 350 nm. The assay was reproducible with overall intra- and inter-day variation of less than 4.8 . The mean recovery of the method was 99.8 ± 2.5 . Using the optimized method, 19 samples were analyzed. The results indicated that the developed HPLC assay could be readily utilized as a quality control method for Hypericum japonicum Thunb.