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Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography

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Rodrigues,  V
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Bülthoff,  I
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Rodrigues, V., & Bülthoff, I. (1985). Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography. Journal of Neuroscience Methods, 13(3-4), 183-190. doi:10.1016/0165-0270(85)90066-4.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-F005-D
Abstract
High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at −76°C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.