Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm 
resolution.

Göttfert, F.; Wurm, C. A.; Müller, V.; Berning, S.; Cordes, V. C.; Honigmann, A.; Hell, S. W.

doi:10.1016/j.bpj.2013.05.029

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Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.

MPG-Autoren

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Göttfert, F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Wurm, C. A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons40296

Müller, V.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Berning, S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Cordes, V. C.
Research Group of Nuclear Architecture, MPI for biophysical chemistry, Max Planck Society;

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Honigmann, A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Zitation

Göttfert, F., Wurm, C. A., Müller, V., Berning, S., Cordes, V. C., Honigmann, A., et al. (2013). Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution. Biophysical Journal, 105(1), L01-L03. doi:10.1016/j.bpj.2013.05.029.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0013-E53F-2

Zusammenfassung

We report on a fiber laser-based stimulated emission-depletion microscope providing down to ~20 nm resolution in raw data images as well as 15–19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.